The Professional Solution for Comprehensive Surface and Air Disinfection

Cold-Fogging Disinfection

The threat of infectious diseases increases steadily world-wide. The dangers of cross-contamination should not be underestimated. A single microorganism can multiply to up to 100 million organisms under favorable conditions within the relatively short time of 12 hours and thus cause great damage. Even after thorough manual disinfection, in most cases, there is still a rather high count of germs.  

The 3-dimensional disinfection procedure is based on a cold-fogging technology. Gaseous hydrogen peroxide (H2O2) destroys the entire spectrum of biological agents (bacteria, virus, fungi, and mold) on dry porous, and nonporous surfaces.

A contribution towards the welfare of your team and your customers, patients, clients and visitors. It safeguards you and your patients or customers against the risk of cross-infection.

Effectiveness through 3-Dimensional Disinfection Procedure

According to the DGKH (German Association for Hospital Hygiene) the efficiency factor by a sole conventional scrubbing and wiping disinfection is only 58%. Consequently 42% of all surfaces and areas are not reached. In addition, the manual disinfection method is subject to some uncertainties:

• Has every surface been treated properly?
• Was the right disinfectant sufficiently used?
• Were clean cloths used?

The 3-dimensional cold-fogging disinfection procedure is the answer to those problems. The evenly distributed disinfectant reaches the most inaccessible gaps, cracks, corners, and edges while all accessible surfaces, equipment, and room air are completely disinfected. Equally important is the fact that it combats a wide range of bacteria (including Legionella), viruses (including Herpes, Hepatitis, HIV, and Influenza), fungi, and molds. This method also minimizes work-related recontamination through the disinfection personnel as employees constantly emit bacteria from their flora, despite protective clothing. Overall, the modern 3-dimensional cold-fogging disinfection method prevents from infections and contaminations. It also protects from dangerous cross-contaminations and even more important it is a highly effective decontamination procedure.

Clinically Proven

The antimicrobial effectiveness of the cold-fogging disinfection method has been assessed and proven by reputable, independent laboratory in numerous studies (e.g. German Society for Hygiene and Microbiology) against many relevant pathogens. It’s been widely used by medical practitioners in Europe in daily practice.

Biocompatible without Toxic Residues

The cold-fogging disinfection is a sustainable and environmentally-friendly procedure through disinfectants which are 99.9% biocompatible. To enhance the disinfection effect and effectiveness silver ions are added. In the last phase of the bio-decontamination process hydrogen peroxide easily breaks down into water and oxygen, leaving no toxic residues behind.

Safe and Reliable

The cold-fogging disinfection is a non-carcinogenic procedure unlike using formaldehyde which is classified as highly carcinogenic by the World Health Organization (WHO). The automatic mist fogging method assures an evenly distribution of the disinfectant which results in an exemplary hygiene quality. The unit also automatically nebulizes the right amount of disinfectant for the appropriate size of the room, which contributes to a very high reliability of the disinfection method. During the low-noise decontamination process the disinfection system is also self-disinfected. After the process there is an immediate verification of disinfection results through the use of peroxide test strips.

Material Compatible

Through the 3-dimensional cold-fogging disinfection procedure all accessible surfaces, equipment, and room air are completely disinfected. It is further effective on dry porous and nonporous surfaces. Even sensitive electronic, electrical equipment, and furniture are not affected or damaged. The high fine, dry mist of gaseous H2O2 also prevents moisture damages. This high material compatibility results in none to low spatial arrangements.

Convenient and Rapid Approach without Interfering Operations

The bio-decontamination process takes around 90 minutes plus additional 45 minutes time for ventilation. There is no re-wiping of the disinfected surfaces needed as there is no damp film left behind. This rapid approach can be easily integrated into the hygiene and quality management and has no big disturbance of daily routine which leads to minimal effort for customers personnel. The system can be modified and adapted to the clients’ specific room layout and requirements.

Wide Spectrum

The 3-dimensional cold-fogging disinfection is used in a wide spectrum of industries:

• Hospitals
• Medical and Dental clinics
• Ambulance vehicles
• Laboratories
• Retirement homes
• Pharmaceuticals
• Food production
• Veterinary

Even large areas can be effectively treated. In addition, it has been proven that this method is also effective against mold charges and yeasts and can be used for odor neutralization.

Functionality of Cold-Fogging Disinfection and Operating Principle

The extremely fine, cold disinfection mist penetrates through the room from all sides, reaching the most inaccessible areas. The disinfectants are based on the combined effect of the two main active ingredients: hydrogen peroxide (H2O2) and silver ions. The Oxygen, being split-off from H2O2, is breaking through the biofilm of the microorganism and allowing the silver (silver is reinforcing and sustainable) to freely kill the bacteria and inactivate the viruses unhindered. This combination potentiates the biocidal activity of the disinfectants, which ensure that microorganisms can be effectively eliminated or inactivated.

Typical Pathogens - Bacteriology & Virology

The 3-dimensional cold-fogging disinfection eliminates bacteria (including Legionella), fungi, and molds as well as inactivates numerous viruses (including Herpes, Hepatitis, HIV, Influenza). Hydrogen peroxide nebulization is rapidly becoming the accepted alternative to formaldehyde for regulatory health and safety agencies. There is a constant review and checking of new pathogens by medical specialists for microbiology and infectious epidemiology as well publicly appointed and sworn experts for hospital hygiene.

“Disinfection errors can have serious consequences. On the other hand, DioProtection is an absolutely safe method of disinfection. With this efficient cold-fogging disinfection method even critical areas are being treated reliably.” (Thomas Naß, State-certified disinfector)

According to the presently available expertise, below mentioned bacteria, fungi and viruses can be successfully eliminated with the DioProtection-System:


Staphylococcus Aureus

Most common pathogen in wound infections, but also numerous other infections.

E. Coli, Klebsiella Sp., Serratia Sp., Citrobacter Sp., Proteus Sp., Enterobacter Sp.

Typical intestinal bacteria.

Staphylococcus Epidermidis and Staphylococcus Sp.

Most common pathogen in wound infections, but also numerous other infections.

Bacillus Sp. (Aerobic Spore Producers)

Apathogenic bacterium. Is often found after disinfection measures.

Streptococcus Sp.

Environmental and skin bacterium, usually with low pathogenicity. However, it can also cause wound infections and other infections.

Enterococci (E. Faecalis, E. Faecium)

Typical intestinal streptococci, usually with low pathogenicity. Indication of faecal contamination.

Haemolytic Streptococci (Group A, B, C, G and F)

Pathogen that causes erysipel, pharyngeal inflammations (including scarlet fever), severe wound infections, and much more.

Pseudomonas Aeruginosa, Pseudomonas Sp., Acinetobacter Sp., Stenotrophomonas Maltophilia

Typical bacteria with a preference for moist locations. Occurs in wash basin waste pipes and taps. Pathogen that causes inflammations of the middle ear, wound infections, urinary tract infections.

Enterohemorrhagic E. coli (EHEC) Bacteria

A special type of coliform bacteria. Some of these pathogens may cause severe hemorrhagic colitis with gradients and hemolytic uremic syndrome (HUS).

Clostridium Difficile

Obligately anaerobic, gram-positive rod-shaped bacterium. Most common pathogen of nosocomial infections often causing violent diarrhea and severe intestinal inflammation.

Serratia Marcescens

Occurs naturally in soil and water and produces a red pigment at room temperature.


The genus Legionella is a pathogenic group of Gram-negative bacteria that includes the species L. pneumophila.


Candida Albicans, Candida Sp. among others

Yeast fungi, that also occur in oral and pharyngeal areas and the intestines. Pathogen that causes oral Candidosis and skin mycoses. Severe infections in immunocompromised patients.


Penicillium a widespread genus that is important in foods. Penicillium species contaminate a wide variety of foods and are capable of growing at refrigerated foods. Thus, they often spoil refrigerated foods, especially cheese. They are also common on grains, breads, cakes, fruits, preserves, cured and aged hams and sausages, and in the spoilage of certain fruits. 


A common mold (a type of fungus) that lives indoors and outdoors. Most people breathe in Aspergillus spores every day without getting sick. The types of health problems caused by Aspergillus include allergic reactions, lung infections, and infections in other organs.


Some molds and yeasts cause disease or food spoilage, others play an important role in biodegradation or in the production of various foods, beverages, antibiotics and enzymes. These spores are small enough that people can actually inhale them deep into the lungs. Inhalation of spores poses risk of developing respiratory problems.


Influenza Virus

Influenza viruses belong to the family Orthomyxoviridae. They are divided into four different types, Influenza virus A, B, C and the Thogoto virus. Influenza viruses damage the mucosa of the respiratory tract and reduce the body's defenses.


Human immunodeficiency virus (HIV) is a lentivirus (belongs to the retrovirus family) that causes acquired immunodeficiency syndrome (AIDS).  Infection with HIV occurs by the transfer of bodily fluids such as blood.

Herpes Simplex Virus (HSV)

Also known as Human herpes virus (HHV) are ubiquitous and contagious and belong to the herpes virus family, Herpesviridae, that infect humans.

Enveloped Viruses

Hepatitis B, Hepatitis C, Hepatitis D and GB viruses.


Is a contagious virus that causes vomiting and diarrhea. Our disinfectants have been successfully tested against norovirus according to European standard EN 14476:2007-02.


The SARS-CoV-2 infected cases have symptoms like fever, fatigue, dry cough, dyspnea etc., with or without nasal congestion, runny nose or other upper respiratory symptoms. The respiratory illness responsible for the COVID-19 pandemic.

Effective and Sustainable Mold Remediation through Gaseous Hydrogen Peroxide Cold-Fogging Disinfection

The microbiologically-tested method provides an appropriate, supplementary procedure for the effective elimination and prevention of molds and fungal contaminations. This environmentally-friendly treatment also eliminates airborne mold spores to prevent a recontamination of surfaces. In addition, the hydrogen peroxide cold-fogging procedure does not leave any annoying odors. Consequently, it has a long-term effect and is a sustainable prevention of health damages.

Publications and Opinions

Expert opinions on using hydrogen peroxide cold-fogging to decontaminate rooms with Diosol disinfectant by private lecturer Dr. med. Andreas Schwarzkopf, who is a medical specialist in microbiology and infection epidemiology as well as a licensed expert for hospital hygiene

Does the atomization of the compound Diosol comply with the requirements of disinfection in a clinical practice?

A review of previous opinions showed that the basic requirements of the German Association of Hygiene and Micro-Biology as well as the Network of Applied Hygiene (VAH) are completely fulfilled with the use of a 3% or 6% solution, respectively. An adequately disinfected effect was achieved even when a test contamination of 0.3% albumin and 0.3% erythrocytes was used. We believed that it would be sensible to test with a stronger contamination in addition to the tests upon which previous expertise’s were based. Therefore, we chose to use a more than triple contamination of 1% each of albumin and erythrocytes. When applied to the surgery room tiles, this resulted in a clearly visible contamination. It is very significant that the system achieves an equivalent reduction factor of 5 (from 100,000 bacteria to one bacterium) of the enterococcus used as test contamination, although it was found in an extreme contamination. Even in the shadow areas a reduction factor of 2 is obtained, corresponding to a normal cleaning. I conclude that the procedure is very effective in the presence of protein quantities commonly encountered in a clinical practice environment, even under aggravated circumstances.

“DioProtection meets the requirements of Biostoff-Verordnung, TRBA 250 as well as the KRINKO/RKI recommendations concerning the demands of hygiene for cleaning and disinfection of surfaces due to a modern aerosol disinfection method.” (PD Dr. med. habil. A. Schwarzkopf)

“A review of previous hygiene studies showed that the requirements of the German Society for Hygiene and Microbiology (DGHM) respectively the Association for Applied Hygiene (VAH) are fully met when using a 3% and a 6% solution.” (PD Dr. med. habil. A. Schwarzkopf)

“This (room decontamination through nebulization) may be an important aspect of infection prevention, especially in the outbreak management.” (PD Dr. med. habil. A. Schwarzkopf)

The Robert-Koch-Institute (RKI) evaluates cold-fogging disinfection based on hydrogen peroxide as positive

“The rapidly advancing automation and electronization of laboratory processes, demands today decontamination processes that are easy to master, are residue-free, conserve equipment and system resources, and are reliable and reproducible in their effect. These requirements seem to be met with the use of H2O2. For this reason, gaseous nebulization of H2O2 has been and is increasingly being used in laboratory stages 3 and 4 as well as in industrial cleanrooms for disinfection purposes.”

“To inactivate viruses, initial concentrations of 5% in combination with a small amounts of silver ions can be used for cold-fogging disinfection even at high air humidity.”

“The basic suitability of the procedure for the disinfection of complex surfaces emerges from the examination and the subsequent acceptance.”

“In summary, it can be stated that the use of a cold-fogging procedure of rooms and ventilation systems for decontamination purposes with H2O2 is an effective and environmentally-friendly alternative to the use of formaldehyde gas.”

Dental Implantology - Protection against Infection through Nebulization
Der Hygieneinspektor - Disease Transmission Can Have Many Causes
Ernährung Aktuell - Room Decontamination with Hydrogen Peroxide Cold-Fogging
Ernährung Aktuell - The H2O2-Based Room Decontamination for Hygienically Perfect Production Environments in the Food Industry
European Hospital - H2O2 Disinfects Air, Surfaces and Rooms
European Hospital - Just Nebulise Germs Away
Management & Krankenhaus - Cold-Fogging of Hydrogen Peroxide
Management & Krankenhaus - Dark Past or Bright Future
Markt und Mittelstand - To the Surface
Oemus Media Verlag - Infection Control through Biocompatible Aerosol Disinfection
Pharma+Food - Efficiency in the Hygiene Process
Rationell Reinigen - Nebulized Pathogens - Disinfection without Residues
Reinigungs Markt - DioProtection in Application - Maximum Safety
Reinigungs Markt - DioProtection-Room Disinfection in Terms of Environmental Hygiene
Reinraum Technik - H2O2-Based Cold-Fogging
Rettungs-Magazin - How Effective is Hydrogen Peroxide Cold-Fogging
Seniorenheim-Magazin - Hydrogen Peroxide Based Room and Surface Disinfection in Elderly Care

Evaluations and Accreditations

Advantages of H2O2 Disinfection vs. Formaldehyde
Certificate from Regional Council of Darmstadt - Diosol 7.9
Daily Disinfection Practice - H2O2 Diosol Disinfection
EN Conformity of Diosol Generator & Disinfectants
European Standards - Chemical Disinfectants & Antiseptics
Robert Koch Institute List of Approved Disinfectant Procedures & Disinfectants - H2O2
Rota & Noroviruses
Status Quo of Room Decontamination by H2O2
Toxicological Report - H2O2 Diosol Disinfection
Validation H2O2 Diosol Disinfection - Ambulance Vehicle
Validation H2O2 Diosol Disinfection - Animal Pathogenic Viruses
Validation H2O2 Diosol Disinfection - Hospital Hygiene
Validation H2O2 Diosol Disinfection - Influenza & Swine Flu
Validation H2O2 Diosol Disinfection - Microbiocidal Inactivation
Validation H2O2 Diosol Disinfection - Murine Norovirus
Validation H2O2 Diosol Disinfection - Sporocidia
Validation H2O2 Diosol Disinfection - TBC
Validation H2O2 Diosol Disinfection - Veterinary Medicine
Validation on Efficacy - H2O2 Diosol Disinfection
Validation on Efficacy of Various Viruses - H2O2 Diosol Disinfection
Validation H2O2 Diosol Disinfection - EN 17272 2020 Aerosol Distribution
Validation H2O2 Diosol Disinfection - EN 17272 2020 Airborne Room Disinfection


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